Mucopolysaccharides in beef retina and small cerebral vessels

On Ecteola columns in 0.05M HCl (pH 1.3) the sulfate groups of mucopolysaccharides are. dissociated while the carboxyl groups are not. In 0.05M acetate buffer (pH 4.8), the carboxyl groups are dissociated and those mucopolysaccharides which do not bind, to Ecteola at the loioer pH can be resolved. These properties have been utilized, to resolve mixtures of beef retina and small beef cerebral blood vessel mucopolysaccharides in order to further characterize and partially identify those mucopolysaccharides which are associated with the nervous elements and those which are associated ivith the blood vessels. These studies reveal that the retina contains a complex mixture of mucopolysaccharides while the small cerebral vessels contain a more clearly defined mixture.